⮝ Full datasets listing
PXD079961
PXD079961 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling of HSP27 knockout effects on ubiquitination, misfolded proteins, and stress granules in U87-MG glioblastoma cells |
| Description | This project aims to systematically investigate the role of HSP27 in maintaining protein homeostasis (proteostasis) in U87-MG human glioblastoma cells. We performed three complementary quantitative proteomics analyses to compare HSP27 knockout (KO) and wild-type (WT) control groups. First, we characterized the ubiquitinome by enriching ubiquitinated peptides using an anti-K-ε-GG antibody to identify changes in ubiquitination sites and pathways. Second, we employed TME (tetramethylrhodamine) labelling coupled with copper-catalyzed click chemistry and streptavidin bead enrichment to profile misfolded proteins. Third, we isolated stress granule (SG) components via differential centrifugation to examine the protein composition of SGs upon HSP27 depletion. All samples were analyzed using LC-MS/MS-based 4D-FastDIA proteomics. This integrative dataset provides a comprehensive resource for understanding how HSP27 coordinates ubiquitin-proteasome pathway, misfolded protein clearance, and stress granule dynamics in glioblastoma cells. |
| HostingRepository | iProX |
| AnnounceDate | 2026-06-18 |
| AnnouncementXML | Submission_2026-06-22_00:52:23.052.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuexia Jiao |
| SpeciesList | scientific name: Human parechovirus strain T82-0169; NCBI TaxID: 1001056; |
| ModificationList | ubiquitination signature dipeptidyl lysine |
| Instrument | timsTOF Pro; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-22 00:51:05 | ID requested | |
| ⏵ 1 | 2026-06-22 00:52:23 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: HSP27, U87-MG, Glioblastoma, Ubiquitination, Stress Granules, Misfolded Proteins, Quantitative Proteomics, 4D-FastDIA, Proteostasis |
Contact List
| Zijiang Tang | |
|---|---|
| contact affiliation | Huazhong Agricultural University |
| contact email | zijiantang@mail.hzau.edu.cn |
| lab head | |
| Yuexia Jiao | |
| contact affiliation | Huazhong Agricultural University |
| contact email | jiao326@webmail.hzau.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
