PXD079953

PXD079953 is an original dataset announced via ProteomeXchange.

Title	A Multiplex MRM Quantitative Method for Forensic Body Fluid Identification
Description	This study was performed based on the Shimadzu LCMS-8050 high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (HPLC-MS/MS) platform. A targeted multiplex peptide detection method utilizing high-flow-rate reversed-phase chromatography was established. Synthetic peptides were adopted to optimize the analytical workflow, and stable isotope-labeled internal standard peptides were introduced to achieve reliable absolute quantification of 21 body fluid-specific peptides. The established peptide absolute quantification strategy was further applied to forensic identification of body fluid and tissue origins. In addition, three types of control peptides were incorporated into the assay. Bovine-derived CYCS protein was used as a negative quality control marker. Matrix samples spiked with bovine serum albumin (BSA) were repeatedly injected to assess the method’s accuracy and precision. Meanwhile, matrix-matched samples prepared from peripheral blood, menstrual blood, vaginal secretions, semen and saliva were analyzed to evaluate matrix effects.
HostingRepository	iProX
AnnounceDate	2026-06-20
AnnouncementXML	Submission_2026-06-21_20:45:32.453.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyuan Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-21 20:44:32	ID requested
⏵ 1	2026-06-21 20:45:32	announced

Dataset with its publication pending

submitter keyword: Body fluid identification, Multiple reaction monitoring (MRM), Absolute quantification, Isotope-labeled peptides

Lei Feng
contact affiliation	Institute of Forensic Science, Ministry of Public Security
contact email	fengleink@163.com
lab head
Zhiyuan Yang
contact affiliation	Institute of Forensic Science, Ministry of Public Security
contact email	yangzy_wz@163.com
dataset submitter

iProX dataset URI