PXD079900

PXD079900 is an original dataset announced via ProteomeXchange.

Title	Glycan bead precipitation of proteins from Toxoplasma gondii lysate
Description	Apicomplexan parasites use two sets of specialized organelles to invade mammalian host cells, the micronemes and the rhoptries. Microneme proteins mediate adhesion and interaction with host cell surfaces and upon appropriate contact mediate discharge of rhoptry organelles. Injection of rhoptry contents into host cells prepares the host cell for invasion and intracellular replication. We discovered that host galactose-containing N-glycans are required for rhoptry discharge from the apicomplexan Toxoplasma gondii, though the corresponding interacting microneme protein was unknown. Here, we identify the parasite proteins able interact with galactose and lactose. We used agarose beads coated with lactose and galactose to precipitating the specific interactors from whole-parasite lysate. Mannose coated beads were used as a negative control due to host mannose being inconsequential in the rhoptry discharge process. The microneme proteins MIC1, MIC4, and MIC6 were identified as the primary strong interactors of both galactose and lactose. Follow up analysis confirmed their importance to rhoptry discharge and parasite invasion via their interactions with galactose-containing N-glycans. This is the first such host-parasite interaction discovered in Toxoplasma to regulate rhoptry discharge.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_13:57:18.012.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD079900
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dylan Valleau
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: NEWT:383379;
ModificationList	phosphorylated residue; monohydroxylated residue; deaminated residue; hexosylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-18 15:38:57	ID requested
⏵ 1	2026-08-18 13:57:18	announced

10.6019/PXD079900;

submitter keyword: Toxoplasma gondii, galactose, mannose, invasion, lactose, microneme

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA
contact email	lourido@wi.mit.edu
lab head
Dylan Valleau
contact affiliation	Whitehead Institute for Biomedical Research
contact email	valleau@wi.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079900
     1. Label: PRIDE project
     2. Name: Glycan bead precipitation of proteins from Toxoplasma gondii lysate