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PXD079853
PXD079853 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lactate-driven lysine lactylation mediates high-level colistin resistance in Salmonella |
| Description | Intracellular pathogens are particularly refractory to antibiotic eradication, leading to persistent and relapsing infections. However, how intracellular metabolic microenvironments shape antibiotic resistance phenotypes remains poorly understood. Here we delineate a metabolic-regulatory axis wherein Salmonella Typhimurium undergoes profound metabolic rewiring upon intramacrophage adaptation, suppressing oxidative phosphorylation and the tricarboxylic acid (TCA) cycle while upregulating anaerobic glycolysis. This metabolic shift generates elevated lactate levels that drive widespread lysine lactylation, including modifications on the two-component system regulators PmrA and PmrD. Lactylation at specific residues stabilizes phosphorylated PmrA, thereby activating lipopolysaccharide modification genes that remodel the outer membrane charge to confer high-level colistin resistance. Critically, metabolic intervention with exogenous citrate restores TCA cycle activity, reduces lactate production and subsequent PmrA/D lactylation, and resensitizes bacteria to colistin both in vitro and in vivo. These findings reveal metabolic reprogramming as a deterministic driver of post-translational regulatory networks governing antibiotic resistance, offering a paradigm for targeting bacterial metabolism to combat drug-resistant bacteria. |
| HostingRepository | iProX |
| AnnounceDate | 2026-06-18 |
| AnnouncementXML | Submission_2026-06-18_00:55:42.800.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shuang Zhou |
| SpeciesList | scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. 798; NCBI TaxID: 1008297; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-18 00:55:21 | ID requested | |
| ⏵ 1 | 2026-06-18 00:55:43 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Antimicrobial resistance, bacteria, colistin, lysine lactylation, metabolism |
Contact List
| Yuan Liu | |
|---|---|
| contact affiliation | Yang Zhou University |
| contact email | liuyuan2018@yzu.edu.cn |
| lab head | |
| Shuang Zhou | |
| contact affiliation | Yang Zhou University |
| contact email | zshuang202408@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
