PXD079815 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | NEK1 autophosphorylation is disrupted by amyotrophic lateral sclerosis-associated missense variants: activity biomarkers and structural insights |
| Description | Rare variants in NEK1, encoding a serine/threonine kinase, are established contributors to amyotrophic lateral sclerosis (ALS), but the biochemical effects of ALS-associated NEK1 missense variants on kinase activity have not been directly investigated. This project maps the phosphorylation landscape of human NEK1 and identifies recurrent phosphorylation sites using cell-based and recombinant phosphoproteomic approaches. GFP-tagged wild-type and kinase-dead NEK1 were analysed in a NEK1 knockout U-2 OS background, and recombinant NEK1/C21ORF2 complexes were also examined. LC-MS/MS phosphosite mapping, targeted extracted ion chromatogram analysis, phosphosite mutagenesis and phosphospecific antibody validation were used to assign pSer14, pThr156 and pSer418 as NEK1 autophosphorylation sites. Activation-loop pThr156 was then used as a readout of NEK1 kinase activity to assess ALS-associated NEK1 missense variants. The dataset from Mehta Lab provides a curated phosphoproteomic resource for NEK1 regulation, kinase activity measurement and functional interpretation of ALS-associated NEK1 variants. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-02 |
| AnnouncementXML | Submission_2026-09-02_05:08:50.427.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Arpan Mehta |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-17 03:21:17 | ID requested | |
| 1 | 2026-09-02 04:36:21 | announced | |
| ⏵ 2 | 2026-09-02 05:08:51 | announced | 2026-09-02: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: NEK1 kinase |
| autophosphorylation |
| amyotrophic lateral sclerosis |
| ALS |
| motor neuron disease |
| phosphoproteomics |
| phosphorylation |
| kinase activity |
| activation loop |
| pThr156 |
| pSer14 |
| pSer418 |
| LC-MS/MS |
| HCD |
| EThcD |
| U-2 OS cells |
| GFP immunoprecipitation |
| kinase-dead mutant |
| missense variants |
Contact List
| Arpan Mehta |
|---|
| contact affiliation | Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee |
| contact email | AMehta001@dundee.ac.uk |
| lab head | |
| Arpan Mehta |
|---|
| contact affiliation | MRC Protein Phosphorylation & Ubiquitylation Unit, Faculty of Life Sciences, University of Dundee |
| contact email | amehta001@dundee.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD079815
- Label: PRIDE project
- Name: NEK1 autophosphorylation is disrupted by amyotrophic lateral sclerosis-associated missense variants: activity biomarkers and structural insights
