PXD079682

PXD079682 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics Reveals EPHA2 and EGFR Activation Following Wnt Pathway Inhibition in Wnt-Driven Tumours
Description	Wnt signalling represses multiple receptor tyrosine kinases (RTKs) in Wnt-high cancers. To identify RTKs responsible for MAPK activation following Wnt pathway inhibition, phosphotyrosine-targeted mass spectrometry was performed on xenograft tumour samples derived from HPAF-II and HCT116 cells. Tumours were treated with the PORCN inhibitor ETC-159 or generated from TCF7L2 knockout cells to achieve pharmacological or genetic suppression of Wnt signalling. Immunoaffinity enrichment of phosphotyrosine-containing peptides followed by LC-MS/MS analysis identified increased phosphorylation of EPHA2, EGFR and ERBB2 upon Wnt inhibition. Functional studies demonstrated that EGFR acts as a convergent mediator linking Wnt inhibition and EPHA2 loss to MAPK activation. These data provide insight into RTK signalling rewiring in Wnt-driven tumours and identify therapeutic vulnerabilities involving EGFR and Wnt pathway co-targeting.
HostingRepository	PRIDE
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-20_03:22:46.645.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Changyuan Hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-14 17:38:03	ID requested
⏵ 1	2026-08-20 03:22:47	announced

Dataset with its publication pending

submitter keyword: Wnt Signalling, EPHA2,Phosphoproteomics, EGFR, Immunoprecipitation-Mass Spectrometry, LC-MS/MS

David M. Virshup
contact affiliation	Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore Department of Pediatrics, Duke University School of Medicine, Durham, NC, USA
contact email	david.virshup@duke.edu
lab head
Changyuan Hu
contact affiliation	Monash University
contact email	changyuan.hu@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD079682

PRIDE project URI

• PRIDE
  1. PXD079682
     1. Label: PRIDE project
     2. Name: Phosphoproteomics Reveals EPHA2 and EGFR Activation Following Wnt Pathway Inhibition in Wnt-Driven Tumours