PXD079616 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Insulin synthesis is sustained by readenylation of its own mRNA. |
| Description | Insulin is an essential regulator of glucose homeostasis in vertebrates, and impairment of its synthesis or action leads to diabetes with severe health complications in humans. It is therefore essential to understand how beta cells control insulin synthesis and secretion, including the transcription, translation and decay of its messenger RNA. Using sequencing-based poly(A) tail length profiling from human tissue, genetic evidence for type 2 diabetes, bulk and single-cell transcriptomics and perturbation experiments, here we find that the insulin mRNA is stabilized through readenylation by noncanonical poly(A) polymerases of the TENT5 family. We show that TENT5 activity is highly specific, promoted by both localization at the endoplasmic reticulum and regulatory sequences within the insulin mRNA and stimulated by glucose. Overall, our findings provide a mechanistic link between the dynamic control of insulin production by beta cells and the direct regulation of insulin mRNA metabolism. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-06 |
| AnnouncementXML | Submission_2026-07-06_07:25:08.807.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Andrea Graziadei |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-12 00:01:44 | ID requested | |
| ⏵ 1 | 2026-07-06 07:25:09 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics, Insulin |
| mRNA metabolism |
| beta cells |
| TENT5 |
| poly(A) tail |
| Type 2 Diabetes.,DIA |
Contact List
| Andrea Graziadei |
|---|
| contact affiliation | Human Technopole |
| contact email | andrea.graziadei@fht.org |
| lab head | |
| Andrea Graziadei |
|---|
| contact affiliation | Human Technopole |
| contact email | andrea.graziadei@fht.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD079616 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD079616
- Label: PRIDE project
- Name: Insulin synthesis is sustained by readenylation of its own mRNA.
