⮝ Full datasets listing
PXD079564
PXD079564 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CellPryme CAR T cell phosphoproteomics |
| Description | CellPryme CART cell phosphoproteomics Proteins were prepared using Sera-Mag SpeedBeads and peptide material subjected to phospho-peptide enrichment using titanium dioxide (TiO2) beads (Titansphere TiO #502075010). Peptide samples were analysed using a Thermo Scientific Vanquish Neo UHPLC system operated in trap-and-elute mode, coupled to an Orbitrap Astral mass spectrometer with an Easy-spray ion source in data-independent (DIA) mode. Peptides were loaded onto a PepMap Neo Trap. Global: Full MS scans were acquired in the Orbitrap at 240,000 resolution, over an m/z range of 380-980, with an automatic gain control (AGC) target of 5 10^6, maximum injection time of 5 ms and 1 microscan. MS/MS spectra were collected in the Astral analyser using 200 isolation windows of 3 m/z width (0 m/z overlap) spanning the same precursor range, maximum injection time of 5 ms and 1 microscan. Fragmentation was performed using higher energy collisional dissociation (HCD) with a normalized collision energy (NCE) of 25%, and fragment ions were detected from m/z 150-2000. The total cycle time was approximately 0.6 s. For phospho-enriched samples, peptides were separated using a 24-min method with micro-flow ramping. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-11 |
| AnnouncementXML | Submission_2026-06-11_16:56:31.313.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | David Greening |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Phospho |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-11 00:26:06 | ID requested | |
| ⏵ 1 | 2026-06-11 16:56:31 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: CAR T cell, immunoregulation, immune response, therapeutic, cancer, Phosphoproteomics, DatasetType:Proteomics |
Contact List
| David Greening | |
|---|---|
| contact affiliation | Baker Heart & Diabetes Institute |
| contact email | david.greening@baker.edu.au |
| lab head | |
| David Greening | |
| contact affiliation | Baker Heart & Diabetes Institute |
| contact email | david.greening@baker.edu.au |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102124/ |
