PXD079562

PXD079562 is an original dataset announced via ProteomeXchange.

Title	Intensity of ion masses determined by intact LC-MS analysis and spectral deconvolution of the QatBCD complex.
Description	QatABCD is a widespread prokaryotic anti-phage defense system comprising four protein components, out of which QatC, a QueC-family protein is the signature component. QueC family proteins are nucleoside biosynthesis enzymes involved in the biosynthesis of queuosine, a 7-deazaguanine derivative. Recently, QueC-family proteins were shown to catalyze a deazaguanylation protein-nucleobase conjugation reaction in type IV CBASS antiphage defense. However, the mechanism of QatABCD, even the function of QatC in this system, remains unknown. Here, we demonstrate that QatBCD forms a complex in which QatD is highly flexible. Crystal structures of the QatBC complex in apo and ATP-bound form support a shared role for QueC-family proteins in targeting protein substrates for N-terminal modification as in type IV CBASS. We show that the QatB N-terminal loop, its binding with QatC and QatC catalytic site are essential for QatABCD defense in vivo, suggesting a modification might occur analogous to CBASS. These findings provide structural and functional insights into QueC-family protein in QatABCD system, suggesting the conserved mechanisms and critical roles of QueC-family in prokaryotic immunity.
HostingRepository	PRIDE
AnnounceDate	2026-08-01
AnnouncementXML	Submission_2026-07-31_19:31:22.096.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zirui Gao
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-11 00:08:05	ID requested
⏵ 1	2026-07-31 19:31:22	announced

Dataset with its publication pending

submitter keyword: Q-tof,QatBCD

Yi Zhang
contact affiliation	State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
contact email	zhangyishirly@hotmail.com
lab head
Zirui Gao
contact affiliation	Beijing University of Chemical Technology, North Third Ring Road 15, Chaoyang District, Beijing, China.
contact email	gzr68693589@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079562
     1. Label: PRIDE project
     2. Name: Intensity of ion masses determined by intact LC-MS analysis and spectral deconvolution of the QatBCD complex.