PXD079527 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Discovery and characterization of an anti-Neisseria gonorrhoeae NGO_1985 monoclonal antibody and cognate antigen |
| Description | Gonorrhea, caused by Neisseria gonorrhoeae (Ng), represents a major global health concern, exacerbated by the lack of an available vaccine and rising antibiotic resistance. Partial protection has been linked to the anti-meningococcal 4CMenB vaccine. Using a Reverse Vaccinology 2.0 approach, memory B cells from 4CMenB-vaccinated volunteers were used to generate monoclonal antibodies (mAbs) cross-reactive against Ng. A specific antibody, mAb_4, was selected for detailed investigation. To identify its target, native Outer Membrane Vesicles (nOMVs) from Ng strains were subjected to immunoprecipitation (IP) with mAb_4, followed by liquid chromatography-mass spectrometry (LC-MS/MS) analysis. The cognate antigen was successfully identified as NGO_1985, an outer membrane biogenesis protein and ortholog of the N. meningitidis GNA_2091 vaccine component. Complementary Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) was further employed to map the specific conformational epitope recognized by the antibody. This study elucidates the molecular basis of 4CMenB cross-protection and provides insights for future vaccine and therapeutic designs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-26 |
| AnnouncementXML | Submission_2026-06-26_02:55:05.264.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mattia Cicogni |
| SpeciesList | scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: NEWT:242231; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-10 09:53:04 | ID requested | |
| 1 | 2026-06-26 02:38:18 | announced | |
| ⏵ 2 | 2026-06-26 02:55:05 | announced | 2026-06-26: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: Reverse vaccinology 2.0, YraP, monoclonal antibodies,Bexsero., NGO_1985, Neisseria gonorrhoeae |
Contact List
| Laura Salvini |
|---|
| contact affiliation | Toscana Life Sciences |
| contact email | l.salvini@toscanalifesciences.org |
| lab head | |
| Mattia Cicogni |
|---|
| contact affiliation | toscana life sciences |
| contact email | m.cicogni@toscanalifesciences.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD079527 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD079527
- Label: PRIDE project
- Name: Discovery and characterization of an anti-Neisseria gonorrhoeae NGO_1985 monoclonal antibody and cognate antigen
