PXD079489

PXD079489 is an original dataset announced via ProteomeXchange.

Title	SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus — DIA Proteomics
Description	Group A Streptococcus (GAS) encodes two paralogs of the Spx transcriptional regulator, SpxA1 and SpxA2, which interact with RNA polymerase to modulate virulence gene expression. SpxA2 is directly regulated by the LiaFSR three-component system in response to antimicrobial peptide stress. To define how SpxA2 influences CovR DNA occupancy genome-wide, we performed chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) targeting CovR in wild-type MGAS10870 (emm3), an isogenic spxA2 deletion mutant (deltaSpxA2), and a LiaSQ146A constitutively active LiaS mutant used as a SpxA2 overexpression surrogate (SpxA2-OE). Differential binding analysis identified 439 sites with significantly altered CovR occupancy between wild-type and deltaSpxA2 (FDR < 0.05), with class-specific enrichment of CovR dimer and monomer binding motifs consistent with the two-class CovR binding model (Horstmann et al. 2023). These data contribute to a multi-omic analysis including RNA-seq, DIA proteomics, and NanoString nCounter that defines the regulatory roles of SpxA1 and SpxA2 in GAS pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-07-13
AnnouncementXML	Submission_2026-07-13_10:37:27.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anthony Flores
SpeciesList	scientific name: Streptococcus pyogenes MGAS315; NCBI TaxID: NEWT:198466;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-09 19:04:19	ID requested
⏵ 1	2026-07-13 10:37:28	announced

Morrison GA, Vega LA, Iglesias MS, Holly K, McDonald WH, Horstmann N, Shelburne SA, Gaddy JA, Flores AR, SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus. bioRxiv, ():(2026) [pubmed]

10.64898/2026.06.19.733365;

submitter keyword: Spx,group A Streptococcus, CovR

Anthony R. Flores
contact affiliation	Vanderbilt University Medical Center
contact email	anthony.flores@vumc.org
lab head
Anthony Flores
contact affiliation	Vanderbilt University Medical Center
contact email	anthony.flores@vumc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD079489

PRIDE project URI

• PRIDE
  1. PXD079489
     1. Label: PRIDE project
     2. Name: SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus — DIA Proteomics