PXD079448 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cyclin Dependent Kinase 4 (CDK4) maintains cell cytoskeleton integrity and behavior in Triple Negative Breast Cancer (TNBC) cells |
| Description | Cyclin-Dependent Kinase 4 (CDK4) is best known for its role in cell cycle progression, but its contribution to cellular architecture is less understood. Here, we identify CDK4 as a regulator of cytoskeletal homeostasis in triple-negative breast cancer (TNBC). CDK4 phosphorylates the RhoGAP Myo9b at serine 1935, enhancing its activity and suppressing RhoA–ROCK–mediated actomyosin contractility. Loss or inhibition of CDK4 disrupts cell morphology, destabilizes the actin cytoskeleton, and increases contractility, alongside collapse of the intermediate filament network, including vimentin disorganization and altered mitochondrial distribution. CDK4-deficient cells also show faster but less directed migration. In vivo, tumors derived from CDK4WT and CDK4KO MDA-MB-231 cells implanted in the mammary fat pad display elevated pMLC levels by IHC, consistent with increased contractility, while proteomic analysis reveals enrichment of cytoskeletal pathways. Transcriptomic analyses (SCAN-B, TCGA, I-SPY2) further show that low CDK4 activity correlates with enhanced EMT, migration, metastasis, and RhoA signaling programs in TNBC. Together, these findings establish CDK4 as an upstream regulator of cytoskeletal integrity, linking actin dynamics, intermediate filament organization, and cell behavior, and reveal a structural role beyond its canonical function in cell cycle control. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-22 |
| AnnouncementXML | Submission_2026-06-22_06:20:57.823.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lluis Fajas |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-09 04:51:31 | ID requested | |
| ⏵ 1 | 2026-06-22 06:20:58 | announced | |
Publication List
Keyword List
| submitter keyword: Actin cytoskeleton,CDK4, cell migration, actomyosin contractility, Myo9b, Triple Negative Breast cancer (TNBC) |
Contact List
| Lluis Fajas Coll |
|---|
| contact affiliation | Center for Integrative Genomics, CIG, UNIL Sorge, CH1015 Lausanne, Switzerland |
| contact email | lluis.fajas@unil.ch |
| lab head | |
| Lluis Fajas |
|---|
| contact affiliation | UNIL |
| contact email | lluis.fajas@unil.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD079448 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD079448
- Label: PRIDE project
- Name: Cyclin Dependent Kinase 4 (CDK4) maintains cell cytoskeleton integrity and behavior in Triple Negative Breast Cancer (TNBC) cells
