PXD079332

PXD079332 is an original dataset announced via ProteomeXchange.

Title	Early cardiomyopathy in prediabetic NDPK-B-deficient mice is associated with remodeling of the mitochondrial O-GlcNAc proteome
Description	Diabetic cardiomyopathy (DCM) is characterized by myocardial remodeling that may al-ready be evident during prediabetes, yet the molecular alterations accompanying these early changes remain poorly understood. The present study examined mouse models of Nucleoside diphosphate kinase (NDPK-B)-deficient prediabetes and streptozoto-cin-induced diabetes using O-GlcNAc-associated proteomic profiling to define stage-specific molecular alterations during the progression from prediabetic to diabetic cardiomyopathy. Both models exhibited increased left ventricular extracellular matrix deposition and impaired diastolic function together with activation of the hexosamine bi-osynthesis pathway. Profiling of O-GlcNAc-associated proteins uncovered extensive re-modeling of the mitochondrial proteome already at the prediabetic stage, with respiratory complex I among the most prominently altered targets, alongside changes in substrate metabolism and inflammatory signaling. In overt DCM, the putative O-GlcNAc proteomic profile was associated with a shift toward wider lipid-dependent metabolic reprogram-ming and remodeling of mitochondrial proteins. These findings identify early remodeling of the mitochondrial O-GlcNAc-associated proteome as a molecular signature of predia-betic cardiomyopathy and highlight respiratory complex I proteins as candidate targets for future mechanistic investigations.
HostingRepository	PRIDE
AnnounceDate	2026-07-28
AnnouncementXML	Submission_2026-07-28_04:31:40.739.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuxi Feng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	electron multiplier; Orbitrap Astral; electrospray ionization

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-05 20:34:59	ID requested
1	2026-06-08 04:41:09	announced
⏵ 2	2026-07-28 04:31:41	announced	2026-07-28: Updated project metadata.

Dataset with its publication pending

submitter keyword: prediabetes

diabetes

NDPK-B

HBP

diabetic cardiomyopathy

mitochondria

Yuxi Feng
contact affiliation	Experimental Pharmacology Mannheim (EPM), European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
contact email	yuxi.feng@medma.uni-heidelberg.de
lab head
Yuxi Feng
contact affiliation	Experimental Pharmacology Mannheim (EPM), European Center of Angioscience, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany
contact email	yuxi.feng@medma.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD079332

PRIDE project URI

• PRIDE
  1. PXD079332
     1. Label: PRIDE project
     2. Name: Early cardiomyopathy in prediabetic NDPK-B-deficient mice is associated with remodeling of the mitochondrial O-GlcNAc proteome