PXD079250

PXD079250 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics Elucidation of Cyclic Lipopeptide Pseudodesmin A-Mediated Biofilm eradication in Vancomycin-Resistant Enterococcus faecalis
Description	In this study, we isolated and purified cyclic lipopeptide PDMA from our in-house strain Pseudomonas tolaasii ZTB4. We evaluated its antibacterial and antibiofilm activities against VREF, demonstrating that PDMA effectively eradicates mature biofilms. To decipher the mechanisms underlying this eradication, we employed an integrated multi-omics approach combining transcriptomics, proteomics, and metabolomics to identify key intracellular targets. This represents the first study to investigate the antibiofilm mechanism of PDMA using multi-omics analysis, along with hemolytic assay to evaluate biosafety for future structure-activity relationship analysis. Our findings highlight PDMA as a promising scaffold, underscoring its potential for structural optimization and application in combating biofilm-related drug tolerance.
HostingRepository	iProX
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-03_19:35:34.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xingmei Gao
SpeciesList	scientific name: Enterococcus faecalis R508; NCBI TaxID: 1134801;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-03 19:35:11	ID requested
⏵ 1	2026-06-03 19:35:34	announced

Dataset with its publication pending

submitter keyword: Pseudodesmin A,Vancomycin-Resistant Enterococcus faecalis,Biofilm,Multi-Omics

Kailing Wang
contact affiliation	Dali University
contact email	kailingw@dali.edu.cn
lab head
Xingmei Gao
contact affiliation	Dali University
contact email	15758099263@163.com
dataset submitter

iProX dataset URI