⮝ Full datasets listing
PXD079227
PXD079227 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The endo-lysosomal system drives lumen formation in a human epiblast model |
| Description | In this study, the endolysosomal dynamics of H9 human embryonic stem cells (hESC) undergoing apicosome formation was examined using a spatial proteomics approach based on the engineered ascorbate peroxidase (APEX) 2, followed by mass spectrometry analysis. In detail, we generated H9 hESC line that express APEX2 fused to Podocalyxin in a doxycycline (DOX) inducible manner, which were then grown in the apicosome forming condition in the presence of DOX. At 30-hr timepoint, proximity biotinylation was performed using our previously established APEX2-labeling strategy, which was followed by TMT labeling and mass spectrometry analysis. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-07-21 |
| AnnouncementXML | Submission_2026-07-21_04:17:36.342.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kenichiro Taniguchi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | TMT6plex reporter+balance reagent acylated N-terminal; L-methionine sulfoxide |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-03 06:53:44 | ID requested | |
| ⏵ 1 | 2026-07-21 04:17:36 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: 6-plex tandem mass tag, human embryonic stem cell, apicosome, PODXL, APEX, proximity biotinylation |
Contact List
| Kenichiro Taniguchi | |
|---|---|
| lab head | |
| Kenichiro Taniguchi | |
| contact affiliation | Medical College of Wisconsin |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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