PXD079110

PXD079110 is an original dataset announced via ProteomeXchange.

Title	Loss of ULK1 Promotes TLR4 Acetylation in Bone Marrow-Derived Macrophages
Description	This study focuses on the regulatory effect of ULK1 (Unc-51-like autophagy activating kinase 1) deficiency on TLR4 (Toll-like receptor 4) acetylation in bone marrow-derived macrophages (BMDMs). To systematically explore the regulatory interplay between ULK1 and TLR4 acetylation, primary BMDMs were isolated from wild-type (WT) and ULK1 knockout (ULK1KO) mice and further induced and differentiated into mature macrophages in vitro. Total cellular proteins were extracted from WT and ULK1-deficient BMDMs, and endogenous TLR4 protein was specifically enriched via immunoprecipitation (IP) using a TLR4-specific antibody. The immunoprecipitated protein samples were subsequently subjected to mass spectrometry (MS) analysis to profile the acetylation modification landscape of TLR4. MS screening results successfully identified distinct acetylation modification sites on TLR4.
HostingRepository	iProX
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-06-01_00:58:22.604.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yafei Chang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-01 00:58:08	ID requested
⏵ 1	2026-06-01 00:58:23	announced

Dataset with its publication pending

submitter keyword: ULK1, TLR4, acetylation, macrophage

Sijia Liang
contact affiliation	Sun Yat-sen University
contact email	liangsj5@mail.sysu.edu.cn
lab head
Yafei Chang
contact affiliation	Sun Yat-sen University
contact email	changyf5@mail.sysu.edu.cn
dataset submitter

iProX dataset URI