PXD079087

PXD079087 is an original dataset announced via ProteomeXchange.

Title	BRCA1 exon 11 binds to TOPBP1 to mediate resection
Description	Approximately 60% of the tumour suppressor protein BRCA1 is encoded by a single exon. Many tumours carry mutations in this exon, often resulting in exon skipping and thus a protein of a severely reduced size. Although none of the well-described protein domains of BRCA1 are encoded by this exon 11, the protein lacking this part shows an hypomorphic activity in homologous recombination. To better understand the function of this large exon, we performed proteomic analyses to identify interaction partners via this part of the protein. Here, we report a DNA damage- and phospho-dependent interaction of TOPBP1 to BRCA1 exon 11. Mechanistically, this interaction is required for BRCA1 exon 11’s role in resection during homologous recombination. In contrast, the interaction is not required for TOPBP1’s role in ATR activation. Our data are an important contribution to better understand this relatively poorly characterized part of the BRCA1 protein.
HostingRepository	PRIDE
AnnounceDate	2026-07-29
AnnouncementXML	Submission_2026-07-29_02:09:28.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-31 05:12:40	ID requested
⏵ 1	2026-07-29 02:09:29	announced

10.64898/2026.01.09.698382;

submitter keyword: BRCA1 exon 11, TOPBP1, DNA end-resection,BRCA1, Homologous recombination

Román González Prieto
contact affiliation	CABIMER University of Sevilla SPAIN
contact email	roman.gonzalez@cabimer.es
lab head
Román González-Prieto
contact affiliation	Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact email	roman.gonzalez@cabimer.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD079087

PRIDE project URI

• PRIDE
  1. PXD079087
     1. Label: PRIDE project
     2. Name: BRCA1 exon 11 binds to TOPBP1 to mediate resection