PXD079061

PXD079061 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of plasma from rescued Kemp’s ridley sea turtles (Lepidochelys kempii) exposed to light and heavy degrees of oiling from the Deepwater Horizon oil spill
Description	In 2010, the Deepwater Horizon oil spill resulted in an estimated loss of 61,000–174,000 sea turtles in the Gulf of Mexico. Rescued Kemp’s ridley sea turtle exhibited adverse effects, including physiological stress and severe derangements. While prior studies evaluated health impacts of oil exposure, effects on plasma proteins remained largely unexplored. This study compared the plasma proteome of turtles from two oiling categories (OC) before and after rehabilitation. Plasma samples were collected from lightly oiled (n=9) and heavily oiled (n=15) turtles at admission and post-rehabilitation. Proteins were analyzed using label-free tandem mass spectrometry. At admission, few differences were observed between groups, with 17 proteins reduced in heavily oiled turtles relative to lightly oiled turtles. After rehabilitation, no significant differences were detected, suggesting normalization of the plasma proteome. Osteocalcin, a bone mineralization and metabolic hormone, displayed a large increase after recovery implicating a mechanistic role for this hormone in the recovery of sea turtles. Unexpectedly, alpha-fetoprotein was the most abundant plasma protein in 41 of 48 samples representing 20.2 ± 3.1% of the molar protein fraction surpassing albumin which is routinely measured for diagnostic evaluation. These findings demonstrate that plasma proteomics can distinguish oiling status and support future biomarker development.
HostingRepository	PRIDE
AnnounceDate	2026-05-29
AnnouncementXML	Submission_2026-05-29_11:12:07.538.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD079061
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Neely
SpeciesList	scientific name: Lepidochelys kempii; NCBI TaxID: NEWT:8472;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-29 10:21:24	ID requested
⏵ 1	2026-05-29 11:12:08	announced

10.6019/PXD079061;

submitter keyword: Kemp’s ridley sea turtle, plasma, LC-MS/MS

Camille Berardone, Benjamin A. Neely, Michael G. Janech
contact affiliation	National Institute of Standards and Technology, NIST - Charleston
contact email	benjamin.neely@nist.gov
lab head
Benjamin Neely
contact affiliation	National Institute of Standards and Technology
contact email	benjamin.neely@nist.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079061
     1. Label: PRIDE project
     2. Name: Proteomic analysis of plasma from rescued Kemp’s ridley sea turtles (Lepidochelys kempii) exposed to light and heavy degrees of oiling from the Deepwater Horizon oil spill