PXD079047

PXD079047 is an original dataset announced via ProteomeXchange.

Title	Complexome profiling of mitochondrial complex and respirasome assembly during cell cycle phase with or without NDUFA3 knockdown
Description	HeLa cells were synchronized for interphase or mitosis. For interphase synchronization, cells were treated with 2 mM thymidine for 19 h and then harvested. For mitotic synchronization, cells were treated with 2 mM thymidine for 19 h, followed by a 9 h release in fresh medium. Mitotic cells were harvested by mitotic shake-off. Mitochondrial membrane fractions were extracted from interphase and mitotic cells using digitonin. Samples were then separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). After electrophoresis, the gel was fixed and stained with Coomassie Brilliant Blue. Each entire lane was excised and cut into 13 sections from top to bottom. Each section was analyzed by LC-MS/MS for complexome profiling.
HostingRepository	iProX
AnnounceDate	2026-05-29
AnnouncementXML	Submission_2026-05-29_01:21:25.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-29 01:21:08	ID requested
⏵ 1	2026-05-29 01:21:25	announced

Dataset with its publication pending

submitter keyword: Complexome profiling

Mitochondrial complex assembly

NDUFA3

Cell cycle

Zhenye Yang
contact affiliation	University of Science and Technology of China
contact email	zhenye@ustc.edu.cn
lab head
Ting Wang
contact affiliation	University of Science and Technology of China
contact email	279219689@qq.com
dataset submitter

iProX dataset URI