PXD079014

PXD079014 is an original dataset announced via ProteomeXchange.

Title	Multiple intron motifs have redundant functions in trans-splicing of the mod(mdg4) locus
Description	In contrast to canonical cis-splicing, trans-splicing combines exons from two distinct transcripts, yielding chimeric mRNAs. One striking example is the ubiquitously expressed modifier of mdg4 (mod(mdg4)) locus in Drosophila, where all mRNAs spanning over 30 isoforms are generated exclusively by trans-splicing. This process combines common constitutive exons with one of many alternative 3′ variable exons transcribed from independent promoters. Here, we demonstrate that trans-splicing is determined by an approximately 600-bp region located in the proximal part of the last common intron. Deletion analysis in a model system and directly within the endogenous mod(mdg4) locus revealed that this intronic region contains multiple redundant motifs for RNA-binding proteins (RBPs), which collectively determine the level of trans-splicing. By affinity purification of the in vitro transcribed proximal part of the intronic RNA, we identified specific binding of a large group of spliceosome-associated RBPs, some of which were found to have binding motifs in the intronic region of mod(mdg4) RNA, which is critical for trans-splicing.
HostingRepository	PRIDE
AnnounceDate	2026-05-28
AnnouncementXML	Submission_2026-05-28_13:29:08.034.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD079014
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-28 13:03:08	ID requested
1	2026-05-28 13:26:09	announced
⏵ 2	2026-05-28 13:29:08	announced	2026-05-28: Updated project metadata.

10.6019/PXD079014;

submitter keyword: RNA motifs,alternative splicing, spliceosome, RNA-binding proteins, trans-splicing, mod(mdg4), Drosophila melanogaster

Georgij Arapidi
contact affiliation	Head of System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD079014

PRIDE project URI

• PRIDE
  1. PXD079014
     1. Label: PRIDE project
     2. Name: Multiple intron motifs have redundant functions in trans-splicing of the mod(mdg4) locus