⮝ Full datasets listing
PXD079006
PXD079006 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | NMNAT2 Loss in Primary Mouse Cortical Neurons at DIV8 and DIV12 |
| Description | Proteinopathy and metabolic dysfunction are central hallmarks of neurodegenerative disease, yet the mechanisms linking these pathologies remain unclear. Here, we investigate the consequences of neuronal nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) loss, a key regulator of NADplus metabolism. We show that NMNAT2 deficiency in neurons results in widespread proteome remodeling of pathways involved in protein quality control and transport, mitochondrial function, and cellular metabolism. WT control and KO neurons were plated on 12-well plates at a concentration of 1x106 cells/well. At DIV8 or 12, cells were rinsed 3x with ice-cold PBS. 300ul of PBS was added to each well and cells were detached from the plate using a sterile cell scraper. 2 wells were combined into 1 sample and centrifuged at 300g for 8 minutes. Cell pellets were rinsed once with ice-cold PBS and centrifuged again at 300g for 6 minutes. Samples were flash frozen in liquid nitrogen and stored at -80 C. 2022_04_80: 6 wildtype neuron sample and 6 NMNAT2 KO neuron samples collected at DIV12 2022_07_134: 4 wildtype neuron samples, 4 NMNAT2 KO neuron samples, 4 wildtype neuron samples treated with 1mM NMN, and 4 NMNAT2 KO neuron samples treated with 10mM NMN. All collected at DIV8 |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-28 |
| AnnouncementXML | Submission_2026-05-28_11:31:03.066.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | TMTpro; Oxidation; Phospho |
| Instrument | Orbitrap Exploris 480; Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-28 10:15:43 | ID requested | |
| ⏵ 1 | 2026-05-28 11:31:03 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: mouse, brain, primary cortical neurons, LC-MS, NMNAT2 KO, DatasetType:Proteomics |
Contact List
| Hui-Chen Lui | |
|---|---|
| contact affiliation | Indiana University |
| contact email | hclu@iu.edu |
| lab head | |
| Amber L. Mosley | |
| contact affiliation | Indiana University School of Medicine |
| contact email | almosley@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000101981/ |
