PXD078983

PXD078983 is an original dataset announced via ProteomeXchange.

Title	Urinary multi-omics reveal non-invasive diagnostic biomarkers in clear cell renal cell carcinoma
Description	Clear cell renal cell carcinoma (ccRCC) is the most common kidney malignancy. Yet, no rapid, non-invasive biomarkers are available for diagnosis or screening. Urine represents an ideal analyte matrix due to its accessibility, low invasiveness, longitudinal sampling and the kidney’s central role in filtration. Here, we integrated proteomic, lipidomic, and metabolomic analyses of urine from ccRCC patients and controls to identify diagnostic biomarkers. Multi-omics profiling revealed urogenital metabolic dysregulation in ccRCC, including increased lipid metabolism, altered mitochondrial respiration signatures, and elevated urinary lipid content. We identified three urinary protein biomarkers: serum amyloid A1 (SAA1), haptoglobin (HP), and lipocalin 15 (LCN15). Using a parallel reaction monitoring mass spectrometry workflow, we developed a rapid and sensitive assay and combined these markers into a diagnostic UrineScore. The UrineScore achieved 96% accuracy in receiver operating characteristic analysis in the discovery cohort and 95% accuracy in an independent validation cohort. Together, these results support the feasibility of multi-omics-guided urinary biomarker discovery and represent a step toward accessible diagnostic platforms for ccRCC.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_03:20:52.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-28 05:30:03	ID requested
⏵ 1	2026-08-18 03:20:53	announced

10.1038/s44321-026-00498-2;

Jonsson G, Oliveira T, Hofmann M, Lemberger U, Stejskal K, Kr, š, š, á, kov, á G, Sakic I, Novatchkova M, Mereiter S, Grabmann G, K, ö, cher T, Koglgruber R, Kikic Z, Camano P, á, ez S, Luna Sanchez B, D, í, ez Nicol, á, s V, Rechberger GN, Z, ü, llig T, Hagelkruys A, Englinger B, Schmidinger M, Penninger JM, Urinary multi-omics reveal non-invasive diagnostic biomarkers in clear cell renal cell carcinoma. EMBO Mol Med, ():(2026) [pubmed]

submitter keyword: Human, LC-MS, Clear Cell Renal Cell Carcinoma, Parallel-Reaction Monitoring (PRM), Proteomics

Tiago Oliveira
contact affiliation	IMBA and Medical University of Vienna
contact email	tiago.oliveira@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078983

PRIDE project URI

• PRIDE
  1. PXD078983
     1. Label: PRIDE project
     2. Name: Urinary multi-omics reveal non-invasive diagnostic biomarkers in clear cell renal cell carcinoma