PXD078963

PXD078963 is an original dataset announced via ProteomeXchange.

Title	Energy-translation coupling limits yeast anaerobic growth
Description	This study employs yeast as a model system to thoroughly investigate the differences in regulatory mechanisms under various nutrient limitation conditions, utilizing a systems biology approach integrated with techniques such as multi-omics analysis.To obtain a comprehensive quantitative landscape of protein expression in (human lung adenocarcinoma), we performed TMT 6-plex–based comparative proteomic analysis. Tumour and adjacent non-tumour tissues (n = 6 pairs) were pulverised under liquid nitrogen and total proteins extracted by phenol–ammonium acetate/methanol precipitation. After reduction, alkylation and acetone precipitation, 50 µg proteins per sample were digested in-solution with trypsin (1:50, w/w, 37 °C, 16 h). Peptides were labelled with TMT 6-plex reagents, pooled and fractionated by high-pH reversed-phase chromatography on an Agilent 1100 HPLC (Zorbax Extend-C18, 15 fractions). LC-MS/MS was carried out on a Q Exactive HF-X (Thermo) in DDA mode. Quantitative analysis was performed with Proteome Discoverer 2.4 using a 1 % FDR threshold at PSM and protein levels. The dataset provides relative abundance information for > 6 000 protein groups across tumour and control tissues, serving as a resource for biomarker and pathway studies in lung adenocarcinoma.
HostingRepository	iProX
AnnounceDate	2026-05-28
AnnouncementXML	Submission_2026-05-28_00:45:37.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yongbo Wang
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-28 00:45:14	ID requested
⏵ 1	2026-05-28 00:45:38	announced

Dataset with its publication pending

submitter keyword: Energy, translation, Saccharomyces cerevisiae

Yingping Zhuang
contact affiliation	School of Biotechnology, East China University of Science and Technology
contact email	ypzhuang@ecust.edu.cn
lab head
Yongbo Wang
contact affiliation	School of Biotechnology, East China University of Science and Technology
contact email	Y12222081@mail.ecust.edu.cn
dataset submitter

iProX dataset URI