PXD078952

PXD078952 is an original dataset announced via ProteomeXchange.

Title	Multi-dimensional Proteome Profiling Decodes Coordinated Adaptation in Heat-Stressed Cells
Description	Cellular adaptation to proteotoxic stress requires rapid, multi-layered reprogramming of gene expression. To systematically decode this process, we implemented a time-resolved, multi-dimensional proteomic strategy that simultaneously profiles steady-state abundance (whole-cell proteome), real-time translation (nascent proteome), translational machinery composition (ribosomal proteome), and rapid signaling (phosphoproteome) in HeLa cells across four time points after acute heat stress. Our high-sensitivity Puromycin-Modified Silica Microsphere-based Nascent Proteomics (PMSNP) method was crucial for capturing immediate translational priorities. Multi-omics integration revealed a staged adaptive cascade. RNA splicing emerged as a central hub under multi-layered regulation: splicing factors were among the earliest translationally upregulated components, while concurrent phosphorylation fine-tuned their activity - an integrated strategy that positions the spliceosome as a rapid-response platform for stress-induced proteome remodeling. Furthermore, we uncovered a translation-coupled secretory response of IL1RAP: its synthesis was rapidly induced, accompanied by increased secretion without corresponding changes in whole-cell abundance, and functional assays established secreted IL1RAP as necessary for heat stress-induced cell migration. Notably, this secretory event coincided temporally and pathway-wise with the enrichment of “Golgi vesicle transport” in the sustained remodeling phase, suggesting it is part of a broader secretory reprogramming. This work provides a dynamic, systems-level blueprint of post-transcriptional adaptation, highlighting the power of integrated multi-dimensional proteomics to dissect how cells rewire protein expression through coordinated translational, abundance, and secretory changes in response to stress.
HostingRepository	iProX
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_19:22:42.482.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiawei Dai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 19:21:32	ID requested
⏵ 1	2026-05-27 19:22:42	announced

Dataset with its publication pending

submitter keyword: Multi-dimensional Proteomics, Heat stress, Nascent proteomics, Splicing reprogramming, IL1RAP

Huali Shen
contact affiliation	Fudan University
contact email	shenhuali@fudan.edu.cn
lab head
Jiawei Dai
contact affiliation	Fudan University
contact email	22111510001@m.fudan.edu.cn
dataset submitter

iProX dataset URI