PXD078948

PXD078948 is an original dataset announced via ProteomeXchange.

Title	A biomimetic proteomic approach for capturing and identifying S. mutans potential adhesion proteins via probiotic membrane-modified nanoparticles
Description	Background: Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Probiotic therapy, such as with Lactobacillus acidophilus (L. acidophilus), represents a promising strategy for caries prevention, partly through co-aggregation with S. mutans via bacterial surface adhesins. However, the specific S. mutans adhesion proteins bound by probiotics remain largely unexplored due to challenges including undefined bait proteins, high background noise, and complex membrane structures. Results: We developed a biomimetic proteomic nanoplatform that integrates L. acidophilus membrane-modified nanoparticles with quantitative proteomics to capture and identify S. mutans surface adhesion proteins. This platform enables high-throughput, affinity-based enrichment without predefined bait proteins. Using this approach, we identified several known adhesion-related proteins alongside previously undefined candidates. These findings were validated through subsequent gene knockout experiments. Conclusions: This study innovatively combines membrane-modified nanotechnology with quantitative proteomics to elucidate interbacterial adhesion proteins. The established platform provides a novel methodological approach for investigating bacterial effector proteins and bacteria-bacteria interactions.
HostingRepository	iProX
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_19:13:36.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lan Du
SpeciesList	scientific name: Streptococcus mutans UA159; NCBI TaxID: 210007; scientific name: Lactobacillus acidophilus; NCBI TaxID: 1579;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 19:13:19	ID requested
⏵ 1	2026-05-27 19:13:36	announced

Dataset with its publication pending

submitter keyword: Bacterial Adhesion Proteins, Lactobacillus acidophilus, Membrane-modified Nanotechnology, Proteomics, Streptococcus mutans.

Liang Chen
contact affiliation	Stomatological Hospital of Chongqing Medical University
contact email	chenliang@hospital.cqmu.edu.cn
lab head
Lan Du
contact affiliation	Stomatological Hospital of Chongqing Medical University
contact email	2022120649@stu.cqmu.edu.cn
dataset submitter

iProX dataset URI