PXD078911 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Enriching Extracellular Vesicles from Human Bone |
| Description | Extracellular vesicles (EVs) are nano-sized membrane-bound structures thought to be secreted by all cells. They have multiple functions but are increasingly recognized as mediators of intercellular communication. Established EV isolation protocols for bodily fluids have been primarily focused on blood with limited insights into methods optimized for EVs from other haematopoietic regions. In this study, we present a protocol for the isolation and enrichment of EVs from human bone marrow (BM) and trabeculae (TRAB). This method employs a validated purification strategy, combining iodixanol density cushion (IDC) ultracentrifugation with size exclusion chromatography (SEC), enabling EV recovery from fresh tissue hours after collection. Importantly, this approach facilitates the enrichment of bone-derived EVs without the need for enzymatic digestion or long-term culture, preserving native EV populations1-3. This protocol offers a valuable tool for researchers investigating EVs derived from the diverse cellular constituents of the bone microenvironment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-27 |
| AnnouncementXML | Submission_2026-05-27_08:22:24.776.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD078911 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Eric Bonneil |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-27 08:11:46 | ID requested | |
| ⏵ 1 | 2026-05-27 08:22:25 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics, human bone,extracellular vesicles |
Contact List
| sheela Abraham |
|---|
| contact affiliation | Queen's University |
| contact email | s183@queensu.ca |
| lab head | |
| Eric Bonneil |
|---|
| contact affiliation | Proteomic Platform |
| contact email | eric.bonneil@umontreal.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078911
- Label: PRIDE project
- Name: Enriching Extracellular Vesicles from Human Bone
