PXD078909

PXD078909 is an original dataset announced via ProteomeXchange.

Title	Zebrafish quantitative proteomics
Description	Food allergies, including α-Gal syndrome (AGS), arise from complex interactions between environmental exposure and host intestinal responses that remain poorly defined. We used quantitative SWATH-MS proteomics to characterize zebrafish gut responses to Ixodes ricinus tick saliva or defined salivary protein fractions and subsequent meat consumption. Tick saliva induced coordinated downregulation of metabolic and redox pathways, indicating reduced intestinal functional capacity rather than a classical inflammatory response. The complete protein fraction generated a more fragmented network, combining metabolic suppression with proteostasis impairment and ectopic expression of vitellogenin-like proteins, suggestive of epithelial reprogramming. Individual fractions produced distinct but convergent phenotypes: F1 drove broad suppression of digestive enzymes and loss of enterocyte function; F4 disrupted proteostasis and epithelial integrity; and F5 induced widespread metabolic and mitochondrial dysregulation. Despite limited protein overlap, all fractions converged on metabolic, redox, proteostatic and epithelial collapse pathways, indicating that similar intestinal outcomes arise through distinct molecular routes. F1-associated induction of alpha-2-macroglobulin and vitellogenins suggest links between tick exposure, α-Gal responses and epithelial remodeling. These proteins may serve as biomarkers of AGS-related intestinal reprogramming, pending further validation. Overall, tick saliva acts as a modular system that reconfigures intestinal homeostasis and increases susceptibility to dietary challenges in an AGS-relevant contest
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_10:24:55.015.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078909
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Margarita Villar
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 07:47:50	ID requested
⏵ 1	2026-05-27 10:24:55	announced

10.6019/PXD078909;

submitter keyword: α-Gal syndrome

epithelial reprogramming

intestinal proteomics

tick saliva

zebrafish model

Margarita Villar
contact affiliation	SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos (IREC, CSIC-UCLM-JCCM), University of Castilla-La Mancha, 13071, Ciudad Real, Spain.
contact email	margaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliation	University of Castilla- La Mancha
contact email	margaritam.villar@uclm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD078909

PRIDE project URI

• PRIDE
  1. PXD078909
     1. Label: PRIDE project
     2. Name: Zebrafish quantitative proteomics