PXD078902

PXD078902 is an original dataset announced via ProteomeXchange.

Title	Identification of Plk1-PBD interacting proteins
Description	This project aims to identify Plk1-PBD interactors potentially involved in DNA repair. We performed unbiased proteomic screens in S and G2 phases. HeLa cells were synchronized by double-thymidine block-and-release, and pull-downs were conducted with GST-Plk1 PBD as bait. GST alone and a phospho-binding-deficient GST-PBD mutant (H538A/K540M) served as negative controls. Proteins were then analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-08-01
AnnouncementXML	Submission_2026-07-31_23:41:19.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shinan Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 05:40:27	ID requested
⏵ 1	2026-07-31 23:41:20	announced

Dataset with its publication pending

submitter keyword: GST pull-down,Plk1, PBD, Identification

Fangwei Wang
contact affiliation	Zhejiang University
contact email	fwwang@zju.edu.cn
lab head
Shinan Zhou
contact affiliation	Zhejiang University
contact email	11907083@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078902

PRIDE project URI

• PRIDE
  1. PXD078902
     1. Label: PRIDE project
     2. Name: Identification of Plk1-PBD interacting proteins