PXD078868

PXD078868 is an original dataset announced via ProteomeXchange.

Title	Quantitative metaproteomic analysis of 13C E. coli spike-in mixtures across dynamic range using Orbitrap Astral DIA LC-MS/MS
Description	This dataset contains label-free DIA LC-MS/MS measurements of controlled bacterial spike-in samples designed to evaluate quantitative performance across a wide dynamic range. The samples consist of defined mixtures of 13C-labeled E. coli peptides spiked into unlabeled backgrounds at different ratios (2%, 5%, and 50%) and dilution levels (1/10 and 1/100), along with blank controls. Each condition includes three biological replicates, resulting in a total of 21 LC-MS/MS runs. The dataset was generated to benchmark the sensitivity, quantitative accuracy, and dynamic range of Orbitrap Astral mass spectrometry for metaproteomic and stable isotope probing applications. The experimental design enables systematic evaluation of isotope labeling detection, quantitative linearity, and performance under varying abundance conditions.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-30_20:36:22.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Xiong
SpeciesList	scientific name: gut metagenome; NCBI TaxID: NEWT:749906;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-26 16:49:57	ID requested
⏵ 1	2026-08-30 20:36:23	announced

10.1186/S40168-024-01866-1;

submitter keyword: 13C labeling, dynamic range, DIA, stable isotope probing, label-free proteomics, benchmarking, Orbitrap Astral,E. coli spike-in, metaproteomics

Chongle Pan
contact affiliation	Stephenson School of Biomedical Engineering, The University of Oklahoma
contact email	cpan@ou.edu
lab head
Yi Xiong
contact affiliation	The School of Biological Sciences, The University of Oklahoma
contact email	yixiong@ou.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078868

PRIDE project URI

• PRIDE
  1. PXD078868
     1. Label: PRIDE project
     2. Name: Quantitative metaproteomic analysis of 13C E. coli spike-in mixtures across dynamic range using Orbitrap Astral DIA LC-MS/MS