PXD078831

PXD078831 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of smart-seq2 and DIA proteomics reveal dynamic formation of the Balbiani body during oocyte development in leopard coral grouper (Plectropomus leopardus)
Description	Membraneless RNA granules are essential for germ cell development, yet their subcellular molecular characteristics remain poorly understood in fish. In this study, the Balbiani body (BB) and perinuclear dense body (PDB) in oocytes of leopard coral grouper were isolated using laser capture microdissection (LCM), followed by integrated smart-seq2 and DIA proteomics analyses. Ultrastructural observations showed that PDB appeared in oogonia and persisted throughout oocyte development, whereas BB formed in primary oocytes and gradually expanded into the cytoplasm. Integrative analysis of smart-seq2 and DIA proteomics revealed relatively limited transcriptional variation within BB but pronounced dynamic changes in PDB, accompanied by clear differences in protein composition between the two structures. Functional enrichment indicated that BB was mainly associated with RNA storage and cytoskeletal organization, whereas PDB was enriched in RNA processing and regulatory pathways. Known germline regulators, including piwi, tdrd family members, and buc, exhibited structure-specific expression patterns. Furthermore, ddx6 and igf2bp3 were identified and validated as candidate markers, showing dynamic redistribution from perinuclear regions to BB during oocyte development. These findings reveal distinct molecular features of BB and PDB and provide new insights into subcellular RNA regulation in fish oocytes.
HostingRepository	iProX
AnnounceDate	2026-05-26
AnnouncementXML	Submission_2026-06-02_02:20:55.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ji Liu
SpeciesList	scientific name: Plectropomus leopardus; NCBI TaxID: 160734;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-25 20:05:30	ID requested
⏵ 1	2026-06-02 02:20:56	announced

Dataset with its publication pending

submitter keyword: Balbiani body, Perinuclear dense body, Laser capture microdissection

Ddx6, Igf2bp3

Xi Wu
contact affiliation	Hainan University
contact email	wuxi577@126.com
lab head
Ji Liu
contact affiliation	Hainan University
contact email	15755884718@163.com
dataset submitter

iProX dataset URI