PXD078816

PXD078816 is an original dataset announced via ProteomeXchange.

Title	Culture Protocol Choice Controls Glioblastoma Tumor Architecture and Identity
Description	The project aimed at proteomic profiling of patient-derived glioblastoma organoids from fragment-based (CUT) and dissociation-based (DIS) approaches of organoid generation. Organoids from multiple patient tumors were cultured under growth factor–free or growth factor–supplemented conditions and compared with matched primary tissue. Both protocols (CUT and DIS) produced technically robust glioblastoma organoids when maintained in their native media. CUT organoids matched the reproducibility of DIS cultures while preserving a broader extracellular matrix repertoire and networks linked to collagen assembly, vascular support, and cell–matrix signaling. DIS cultures were biased toward exogenous basement membrane components and proliferative, growth factor–responsive states. Across tumors, CUT organoids consistently showed greater proteomic similarity to matched primary tissue, retaining neural, glial, stromal, and extracellular features largely absent from DIS models.
HostingRepository	PRIDE
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_04:06:34.420.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Václav Pustka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-25 06:51:20	ID requested
⏵ 1	2026-08-13 04:06:35	announced

10.1111/BPA.70125;

submitter keyword: organoids, proteomics,Glioblastoma, dissociation based organoids, fragment based organoids

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Václav Pustka
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	vaclav.pustka@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078816

PRIDE project URI

• PRIDE
  1. PXD078816
     1. Label: PRIDE project
     2. Name: Culture Protocol Choice Controls Glioblastoma Tumor Architecture and Identity