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PXD078800
PXD078800 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DARTS-MS chemoproteomic profiling of ginkgolide J-associated protease-susceptibility candidate proteins in rat PASMC lysates |
| Description | This dataset contains DIA-MS raw files and processed DIA-NN results from a DARTS-MS chemoproteomic experiment designed to identify ginkgolide J-associated protease-susceptibility candidate proteins in primary rat pulmonary artery smooth muscle cell (PASMC) lysates. The experiment included three groups with three biological replicates per group: DMSO vehicle control, protease-only group, and ginkgolide J plus protease group. PASMC protein lysates in the ginkgolide J plus protease group were incubated with ginkgolide J at 25 μM before limited proteolysis with Pronase E. DARTS-MS was used as a target-discovery chemoproteomic approach to detect ligand-associated changes in protease susceptibility rather than conventional steady-state protein expression changes. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-07-19_20:32:13.524.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiacheng Hu |
| SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
| ModificationList | N-acetylated residue; oxidation; S-carboxamidomethyl-L-cysteine |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-25 01:44:33 | ID requested | |
| ⏵ 1 | 2026-07-19 20:32:14 | announced |
Publication List
| Hu J, Qiao Y, Lei G, Gai X, Xia Q, Hu Q, Sun H, Wang H, Li Z, Zhao Y, Wang J, Against Hypoxic Pulmonary Hypertension. Int J Mol Sci, 27(13):(2026) [pubmed] |
Keyword List
| submitter keyword: DARTS-MS |
| DIA-MS |
| chemoproteomics |
| ginkgolide J |
| PASMC |
| Rattus norvegicus |
| Pronase E |
| protease susceptibility |
| pulmonary vascular remodeling |
Contact List
| Xiangyun Gai | |
|---|---|
| contact affiliation | Qinghai Minzu University |
| contact email | 2015002@qhmu.edu.cn |
| lab head | |
| Jiacheng Hu | |
| contact affiliation | Qinghai Minzu University |
| contact email | huxiansheng621@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
