PXD078770

PXD078770 is an original dataset announced via ProteomeXchange.

Title	Implementation of Non‑Isobaric TMT Analogues for Accurate Precursor‑Level Quantification by plexDIA
Description	Multiplexed Data-Independent Acquisition (plexDIA) aims to bridge the gap between the quantitative depth of DIA and the sample throughput of isobaric tag-based workflows. Here, we implement non‑isobaric sample multiplexing using commercially available, isotopically differentiated tandem mass tag (TMT) analogues (TMTpro Zero, standard, and super heavy) to perform plexDIA. By introducing defined mass offsets, we avoid reporter ion interference and we shift signal deconvolution to the MS1 and precursor fragment levels. We benchmarked this workflow using the yeast triple knockout (TKO) standard (Δmet6, Δpfk2, Δura2) analyzed on an Astral mass spectrometer. Notably, targeted quantification closely mirrored biological ground truth, with protein knockouts exhibiting near‑zero intensities strictly within their assigned labeling channels and no detectable cross‑channel signal bleeding. Ultimately, non-isobaric plexDIA avoids the pitfalls of isobaric ratio compression, and provides a highly accurate and scalable framework for quantitative proteomics.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:48.463.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Astral; Orbitrap Exploris 480; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-23 18:54:37	ID requested
⏵ 1	2026-09-07 04:21:49	announced

Wei TY, Paulo JA, Implementation of Nonisobaric TMT Analogs for Accurate Precursor-Level Quantification by plexDIA. Rapid Commun Mass Spectrom, 40(20):e70146(2026) [pubmed]

10.1002/rcm.70146;

submitter keyword: plexDIA, TKO standard, non-isobaric multiplexing, quantitative proteomics

Joao A. Paulo
contact affiliation	Department of Cell Biology Harvard Medical School Boston, MA 02115
contact email	joao_paulo@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078770
     1. Label: PRIDE project
     2. Name: Implementation of Non‑Isobaric TMT Analogues for Accurate Precursor‑Level Quantification by plexDIA