PXD078690

PXD078690 is an original dataset announced via ProteomeXchange.

Title	Alpha-synuclein fibrils induce budding of mitochondrial-derived vesicles
Description	α-synuclein (α-syn) aggregation is a hallmark of synucleinopathies, a class of neurodegenerative disorders such as Parkinson’s disease (PD). Several lines of evidence indicate the involvement of mitochondria in the disease pathology. Despite extensive study, the link between α-syn aggregation and mechanisms of mitochondrial toxicity remains not fully understood. Using high-resolution imaging with electron microscopy, we examined SH-SY5Y cells exposed to α-syn fibrils vs control cells with a focus on mitochondria. We found that upon exposure to α-syn fibrils, mitochondria cristae structure gets defects, and mitochondria enhance the budding of mitochondrial-derived vesicles (MDVs). MDV formation reflects an evolutionarily conserved mechanism reminiscent of bacterial outer membrane vesicle biogenesis. Structural proteomics analysis by mass spectrometry corroborates this microscopy observation by identifying changes in multiple proteins that regulate cristae structure, MDV formation, and trafficking. Our results suggest that α-syn may promote MDV generation, and support an important link between α-syn and mitochondria which will be important for future mechanistic studies. The processes we detected could be of interest for diagnostics and potential therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:28:41.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tetiana Serdiuk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-21 14:06:58	ID requested
⏵ 1	2026-06-28 16:28:41	announced

10.1073/pnas.2604082123;

Braun T, Reber V, Tiberi C, Fr, ä, nkl A, Ghosh D, Riek R, Serdiuk T, Alpha-synuclein fibrils induce budding of mitochondrial-derived vesicles. Proc Natl Acad Sci U S A, 123(24):e2604082123(2026) [pubmed]

submitter keyword: None

Thomas Braun
contact affiliation	Biozentrum, University of Basel, Switzerland.
contact email	thomas.braun@unibas.ch
lab head
Tetiana Serdiuk
contact affiliation	ETH Zurich
contact email	tetiana.serdiuk@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD078690

PRIDE project URI

• PRIDE
  1. PXD078690
     1. Label: PRIDE project
     2. Name: Alpha-synuclein fibrils induce budding of mitochondrial-derived vesicles