PXD078661

PXD078661 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic profiling of SGPP-binding proteins
Description	S-guanylation is an endogenous post translational modification that has been implicated as a specific autophagic signal, yet its molecular mechanism and the identity of its binding proteins remain largely unknown. Here, we developed the first diazirine based photoaffinity probe for S-guanylation (SGPP) to capture and identify its interacting proteins in living cells. The SGPP efficiently labeled target proteins in a time , concentration , and UV crosslinking dependent manner, as demonstrated by in gel fluorescence scanning. Competitive labeling with excess FBnG, a known AUTAC warhead, confirmed the specificity of the probe. Using a chemoproteomic workflow combining SGPP mediated photocrosslinking, affinity enrichment, and mass spectrometry, we identified candidate binding proteins in HeLa cells
HostingRepository	iProX
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_00:40:26.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guanghe Zhu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-21 00:40:02	ID requested
⏵ 1	2026-05-21 00:40:27	announced

Dataset with its publication pending

submitter keyword: S guanylation, photoaffinity probe, chemical proteomics, autophagy, AUTAC, binding proteins

Han Wei
contact affiliation	Zhengzhou University
contact email	weihan548@126.com
lab head
Guanghe Zhu
contact affiliation	Zhengzhou University
contact email	15938824899@163.com
dataset submitter

iProX dataset URI