PXD078621

PXD078621 is an original dataset announced via ProteomeXchange.

Title	XL-MS analysis of recombinant full-length human FHL2
Description	Four-and-a-half LIM domains protein 2 (FHL2) is a modular adaptor protein composed entirely of LIM domains that mediate protein–protein interactions linking the cytoskeleton to transcriptional regulation. In this study, full-length human FHL2 was recombinantly expressed in Escherichia coli and purified without affinity tags. The protein was biophysically and structurally characterized using size-exclusion chromatography coupled to static light scattering (SEC-SLS), small-angle X-ray scattering (SEC-SAXS), and chemical cross-linking coupled to mass spectrometry (XL-MS). Integrative structural analysis based on SAXS and XL-MS data was used to investigate the conformational landscape of FHL2 in solution, revealing the presence of both extended and compactly bent conformations.
HostingRepository	PRIDE
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-09-01_07:38:16.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-20 08:03:16	ID requested
⏵ 1	2026-09-01 07:38:16	announced

10.1016/J.YJSBX.2026.100159;

submitter keyword: Human,FHL2, XL-MS, LIM domain, zinc finger, BS3

Aljaž Gaber
contact affiliation	University of Ljubljana, Faculty of Chemistry and Chemical Technology, Večna pot 113, SI-1000 Ljubljana, Slovenia
contact email	aljaz.gaber@fkkt.uni-lj.si
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD078621

PRIDE project URI

• PRIDE
  1. PXD078621
     1. Label: PRIDE project
     2. Name: XL-MS analysis of recombinant full-length human FHL2