⮝ Full datasets listing
PXD078588
PXD078588 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Reversible perturbation of the intestinal barrier–microbiota–immunity axis and nutritional homeostasis by a short-term gluten-free diet |
| Description | Label-free quantitative proteomic profiling of duodenum tissue from female Balb/c mice subjected to a 3-week dietary intervention. Mice were randomly assigned to a Control group fed a standard gluten-containing diet (group a, n=6) or a Deficiency group fed a nutritionally matched gluten-free diet (group b, n=6), and sacrificed at week 3. Duodenal proteins were extracted in RIPA buffer, digested with trypsin, and analyzed by online nanoLC-MS/MS on a Bruker timsTOF mass spectrometer coupled to a nanoElute UHPLC system, operating in data-independent acquisition (DIA) mode with parallel-accumulation–serial-fragmentation (dia-PASEF). Raw .d data were processed with against the UniProt Mus musculus Swiss-Prot reference proteome (17,252 entries, downloaded 2026-05-18) with peptide and protein FDR controlled at 1%. Differentially expressed proteins were defined as |log2 fold change| >= 1 with Benjamini–Hochberg adjusted P < 0.05. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-19 |
| AnnouncementXML | Submission_2026-05-20_00:48:40.054.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ke Yue |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-20 00:47:54 | ID requested | |
| ⏵ 1 | 2026-05-20 00:48:40 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: gluten-free diet, intestinal barrier, gut microbiota, TLR4/NF-kB, tight junction, mouse duodenum, DIA proteomics, timsTOF, dia-PASEF, food science |
Contact List
| Na Wang | |
|---|---|
| contact affiliation | International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University |
| contact email | na-wang@163.com |
| lab head | |
| Ke Yue | |
| contact affiliation | Henan Agricultural University |
| contact email | yueke202201@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
