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PXD078572
PXD078572 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Glycine-Induced Cargo Alterations of Bacterial Outer Membrane Vesicles by Multi-Omics Analysis |
| Description | Escherichia coli Nissle 1917 (EcN) is a well-characterized probiotic with therapeutic potential in intestinal inflammatory disorders. Its secreted outer membrane vesicles (OMVs) are key mediators of host-microbe interactions and serve as promising platforms for vaccine development and drug delivery. Although glycine is known to enhance OMVs biogenesis in EcN, the resulting cargo alterations are less studied, which are of great importance for the downstream drug delivery and biomedical applications. In this study, we systematically explored the impacts of glycine on the physicochemical properties, yield, and molecular composition of OMVs via morphological characterization, non-targeted and stable isotope-tracing lipidomics. Results showed that glycine stimulation significantly increased the yield of OMVs, particle size, colloidal stability, and purity, while inhibiting bacterial growth. Lipidomic profiling revealed that glycine remodels the lipid composition of OMVs, particularly affecting glycerophospholipid and sphingolipid metabolism. Stable isotope tracing with D₃-serine demonstrated a metabolic shift in phospholipid synthesis from bacterial biomass toward OMVs production. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-19 |
| AnnouncementXML | Submission_2026-05-19_17:43:34.512.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiulei Qi |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-19 17:43:13 | ID requested | |
| ⏵ 1 | 2026-05-19 17:43:35 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Escherichia coli Nissle 1917, outer membrane vesicles, glycine, lipidomics |
Contact List
| Lianghai Hu | |
|---|---|
| contact affiliation | Jilin University |
| contact email | lianghaihu@jlu.edu.cn |
| lab head | |
| Xiulei Qi | |
| contact affiliation | Jilin University |
| contact email | 1152694074@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
