PXD078568

PXD078568 is an original dataset announced via ProteomeXchange.

Title	Systematic Discovery of Pathogen Effector Functions across Human Pathogens and Pathways
Description	Pathogens deploy effector proteins to exploit host cell biology, yet most pathogen open reading frames (ORFs) are rapidly evolving and lack functional annotation. To address this, we developed the eORFeome, a scalable functional genomics platform encompassing 3,835 effector ORFs from diverse viruses, bacteria, and parasites. We screen for modulators of human signalling pathways and this PRIDE deposit contains the supporting mass spectrometry datasets, including AP‑MS, BioID, and whole‑cell MS for selected hits such as U14 (HHV6A) and Rave (Legionella).
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:48.100.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-19 15:16:56	ID requested
⏵ 1	2026-09-07 04:21:48	announced

Pachano T, Leng H, Dugied G, Tribble T, Loubiere V, Lee Y, Rauh F, Manon V, Yuan K, Nurtanto J, Schleiffer A, Young V, Weller B, Lyons EA, Hass MR, Kottyan LC, Weirauch MT, Fuxman Bass JI, Newton HJ, Ensminger AW, Falter-Braun P, Chen J, Schramek D, Stark A, Taipale M, Systematic discovery of pathogen effector functions across human pathogens and pathways. Cell, 189(17):5429-5448.e14(2026) [pubmed]

10.1016/j.cell.2026.06.017;

submitter keyword: pathogens, functional genomics, human signalling pathways,Effectors, host-pathogen interaction, ORFeome, high-throughput screening

Alexander Stark
contact affiliation	IMP, Vienna Biocenter, Vienna, Austria
contact email	alexander.stark@imp.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD078568

PRIDE project URI

• PRIDE
  1. PXD078568
     1. Label: PRIDE project
     2. Name: Systematic Discovery of Pathogen Effector Functions across Human Pathogens and Pathways