PXD078552

PXD078552 is an original dataset announced via ProteomeXchange.

Title	Deuterium oxide labeling methods for bulk and single muscle protein synthesis measures, and measures of integrated muscle protein synthesis and breakdown rates
Description	This methodological pilot study explored the feasibility of an integrated tracer approach for simultaneous measurement of human skeletal muscle protein synthesis and breakdown during prolonged deuterium oxide (²H₂O) labelling. Four healthy men completed a five week protocol combining daily oral ²H₂O intake with selected stable isotope amino acid tracers to assess bulk fractional synthesis rates, fractional breakdown rates, and single protein turnover. The study specifically addressed the methodological question whether bulk or individual protein synthesis measurements remain consistent over a prolonged 2H₂O labelling period. We demonstrate that measurements of mixed-protein fractions in muscle are vulnerable to error due to the rise-to-plateau kinetics associated with longer-duration labelling. Mean mixed-protein synthesis rates can be underestimated when labelling exceeds approximately four weeks. Increasing precursor enrichment mitigated this effect but highlights the need for controlled enrichment strategies in long term studies. In contrast, dynamic proteome profiling (DPP) provided more robust single protein turnover estimates across the full period because the different isotopic plateaus of individual peptides could be modelled. The study also tested the feasibility of combining ²H₂O labelling with ¹⁵N alanine–based breakdown measurements and evaluated potential interference from multiple tracers on DPP outputs. Together, these findings outline practical considerations, limitations, and feasibility of an integrated multi tracer framework for studying human muscle protein turnover over extended periods.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:54.142.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078552
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jatin Burniston
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-19 07:02:54	ID requested
⏵ 1	2026-09-07 04:21:55	announced

10.6019/PXD078552;

10.14814/phy2.71013;

H, ø, jfeldt G, Helge BW, van Hall G, Schjerling P, Kj, æ, r M, Mackey AL, Burniston JG, Agergaard J, O labeling methods for bulk and single muscle protein synthesis measures, and measures of integrated muscle protein synthesis and breakdown rates: A pilot study. Physiol Rep, 14(13):e71013(2026) [pubmed]

submitter keyword: Human, skeletal muscle, protein trunover

Prof Jatin G Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
lab head
Jatin Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD078552

PRIDE project URI

• PRIDE
  1. PXD078552
     1. Label: PRIDE project
     2. Name: Deuterium oxide labeling methods for bulk and single muscle protein synthesis measures, and measures of integrated muscle protein synthesis and breakdown rates