PXD078472

PXD078472 is an original dataset announced via ProteomeXchange.

Title	Antibody-Free Profiling of Lysine Dimethylation via Chemoselective OxidationCoupled Reversible Enrichment
Description	Lysine dimethylation (Kme2) is a critical post-translational modification (PTM), while its comprehensive analysis remains challenging due to low stoichiometry and lack of pan-specific antibodies. Here, we developed an antibody-free proteomics strategy combining selective fluorine-mediated oxidation with reversible hydrazide enrichment. In this approach, Kme2 residues were oxidized to aldehyde, covalently captured by hydrazine-functionalized beads, and reversibly released for MS analysis. This approach achieved high specificity and reproducibility, identifying 293 and 284 site-specific Kme2 events in HeLa and A549 cells, respectively. Based on the excellent reproducibility, quantitative analysis enabled precise characterization of Kme2 sites sensitive to the pan-methylation inhibitor Adox. Applied to breast cancer models, the approach revealed cell-type-specific Kme2 signatures: metastatic MDA-MB-231 cells exhibited cytoskeletal and metabolic adaptation Kme2 events, while proliferative MCF-7 cells prioritized translational machinery components. Our antibody-free platform provides a robust tool for Kme2 mapping and methylation-targeted drug discovery, offering insights into epigenetic regulation in cancer progression.
HostingRepository	iProX
AnnounceDate	2026-05-18
AnnouncementXML	Submission_2026-05-18_02:03:18.594.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-18 02:02:56	ID requested
⏵ 1	2026-05-18 02:03:19	announced

Dataset with its publication pending

submitter keyword: Lysine dimethylation, Chemical proteomics, Selectfluor oxidation, Reversible enrichment, post-translational modifications

Hongqiang Qin
contact affiliation	State Key Laboratory of Fine Chemicals, Department of Pharmaceutical Engineering, School of Chemical Engineering, Dalian University of Technology
contact email	qinhq@dlut.edu.cn
lab head
Qi Li
contact affiliation	Northeastern University
contact email	19526537775@163.com
dataset submitter

iProX dataset URI