PXD078427

PXD078427 is an original dataset announced via ProteomeXchange.

Title	Plasticity of hepatic metabolism in Arctic char (Salvelinus alpinus) in response to cyclic hypoxia
Description	The emergence of cyclic hypoxia puts aquatic organisms’ homeostasis under significant strain. Energetic metabolism as well as protein synthesis and folding are particularly altered during hypoxia, while reoxygenation imposes an oxidative challenge. Currently, little is known about how hypoxia-sensitive organisms respond to large oxygen fluctuations. Our previous work on Arctic char revealed that this salmonid, despite being strongly affected by acute hypoxia and reoxygenation (H/R), can successfully reestablish homeostasis, notably through adjustments to hepatic mitochondrial metabolism. However, the mechanisms underlying this acclimation remain poorly understood. We hypothesized that Arctic char remodel their hepatic proteome to optimize energy metabolism, reorganize oxygen-demanding pathways, and maintain cellular homeostasis during repeated H/R cycles. By exposing Arctic char to two or fifteen days of diel cyclic hypoxia, we confirmed this species’ limited capacity to respond to acute H/R. Nevertheless, after fifteen cycles, Fish adjusted their energetic metabolism through coordinated regulation of carbohydrate and lipid pathways and upregulation of amino acid metabolism. Mitochondrial metabolism was strongly reorganized, particularly at the ubiquinone–Complex III interaction level, alongside adjustments in proline utilization and protein processing. Moreover, protein processing and folding pathways were stimulated in both mitochondria and the endoplasmic reticulum. However, chronic cyclic hypoxia may still promote non-mitochondrial ROS production, DNA replication stress, and impaired immune function. This study highlights how a hypoxia-sensitive fish progressively reorganizes its metabolism and oxygen-demanding pathways to establish a phenotype adapted to chronic cyclic hypoxia, while also revealing the physiological costs associated with this acclimation.
HostingRepository	PRIDE
AnnounceDate	2026-06-10
AnnouncementXML	Submission_2026-06-10_15:30:20.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Loïck Ducros
SpeciesList	scientific name: Salvelinus alpinus; NCBI TaxID: NEWT:8036;
ModificationList	TMTpro
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-15 19:47:06	ID requested
⏵ 1	2026-06-10 15:30:21	announced

10.1016/J.CBD.2026.101883;

submitter keyword: cyclic hypoxia, acclimation, oxygen,fish, liver, proteome

Simon G. Lamarre
contact affiliation	Département de Biologie, Université de Moncton, Moncton (NB, Canada)
contact email	simon.lamarre@umoncton.ca
lab head
Loïck Ducros
contact affiliation	Université du Québec à Rimouski
contact email	loick.ducros@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078427
     1. Label: PRIDE project
     2. Name: Plasticity of hepatic metabolism in Arctic char (Salvelinus alpinus) in response to cyclic hypoxia