⮝ Full datasets listing
PXD078423
PXD078423 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A quantitative proteomics dataset for assessment and prediction of low dose X-ray radiation exposure in mice |
| Description | Ionizing radiation induces molecular responses that may be used to estimate exposure when physical dosimeters are unavailable. Here we present two large-scale proteomics datasets generated from mouse dorsal skin punch samples collected following controlled X-ray exposures spanning multiple doses, dose rates, and post-exposure time points. Experiment 1 comprised 96 samples (including 16 reference samples) collected 6 days after exposure to 0–75 cGy delivered at either 30 or 300 cGy/min. Experiment 2 comprised 936 samples (including 236 reference samples) exposed to 0–100 cGy at either 3 or 28 cGy/min dose rates and harvested between 7 and 150 days post-exposure. All samples were processed using a standardized workflow involving automated bead-based digestion and data-independent acquisition mass spectrometry. The datasets include multiple pooled reference sample types, process controls, and system suitability standards ensuring high quality data. All data presented are available via ProteomeXchange at several levels of processing, from raw files through normalized peptide- and protein-level abundance matrices suitable for biomarker discovery and machine learning applications. This dataset will facilitate generation of new insights into the biological changes and molecular signatures resulting from X-ray exposure in mice and may also help inform future studies in humans. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2026-05-22 |
| AnnouncementXML | Submission_2026-05-22_17:53:04.993.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Alex Zelter |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | Orbitrap Eclipse; Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-15 16:28:27 | ID requested | |
| 1 | 2026-05-18 17:39:28 | announced | |
| ⏵ 2 | 2026-05-22 17:53:05 | announced | : Added publication |
Publication List
| Zelter A, Riffle M, Merrihew GE, Mutawe B, Shulman N, Sanders JA, Noble WS, Johnson Erickson DP, Morimoto A, Shaver BA, Steins TN, Cao N, Ford EC, Rudnick PA, Chelsky D, Wan KH, Inman JL, Chang H, Snijders AM, Mao JH, Celniker SE, De Chant J, Obst-Huebl L, Nakamura K, Wu CC, MacCoss MJ. A quantitative proteomics dataset for assessment and prediction of low dose X-ray radiation exposure in mice. bioRxiv. 2026:2026.05.18.725951. doi:10.64898/2026.05.18.725951 |
Keyword List
| submitter keyword: Radiation biodosimetry, data-independent acquisition, DIA proteomics, mouse skin, X-ray exposure, low-dose radiation, biomarker discovery, mass spectrometry, batch correction, machine learning |
Contact List
| Michael MacCoss | |
|---|---|
| contact affiliation | Department of Genome Sciences, University of Washington |
| contact email | maccoss@uw.edu |
| lab head | |
| Alex Zelter | |
| contact affiliation | Department of Genome Sciences, University of Washington |
| contact email | azelter@uw.edu |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
