PXD078377

PXD078377 is an original dataset announced via ProteomeXchange.

Title	Proteoform analysis by mass spectrometry reveals post-translational processing of legumins and vicilins in chickpeas (Cicer arietinum L.)
Description	The most abundant proteins in pulse seed, including chickpea (Cicer arietinum L.), are the 7S globulins (vicilins) and 11S globulins (legumins), which play key roles in plant reproduction and human nutrition and are also recognized as potential allergens. During seed maturation, legumins and vicilins undergo limited proteolysis. Legumins are processed into α- and β-polypeptide chains that remain linked by a disulfide bond, whereas vicilins are cleaved at two sites, generating three lower-molecular-mass polypeptides (α, β, and γ chain). An integrated mass spectrometry approach, combining bottom-up and top-down strategies, was applied to characterize the post-translational processing of legumins and vicilins. Our data suggest that: (i) proteolytic cleavages in both legumins and vicilins occur at conserved sequence motifs; (ii) legumin processing is catalyzed by an asparaginyl endopeptidase, whereas vicilin processing is mediated by legumains, a family of cysteine endopeptidases; and (iii) one chickpea vicilin is N-glycosylated and carries a high-mannose oligosaccharide side chain. Limited proteolysis process represents a key physiological mechanism regulating storage protein assembly, stability, and mobilization during seed development and germination while preserving storage functions. Glycosylation of vicilins further modulates their structural and functional properties, although it may also influence digestibility and allergenic potential.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:09:22.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vincenzo Cunsolo
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: NEWT:3827;
ModificationList	dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-14 15:20:07	ID requested
⏵ 1	2026-05-31 16:09:23	announced

Di Francesco A, Lanzoni A, Pittal, à MGG, Saletti R, Jensen ON, Cunsolo V, Proteoform analysis by mass spectrometry reveals post-translational processing of legumins and vicilins in chickpeas (Cicer arietinum L.). Anal Bioanal Chem, ():(2026) [pubmed]

10.1007/s00216-026-06577-0;

submitter keyword: Orbitrap® mass spectrometry, legumins and vicilins, bottom-up and top-down approaches,Chickpea proteins proteolysis, N-glycosylated plant proteins

Vincenzo Cunsolo
contact affiliation	Laboratory of Organic Mass Spectrometry, Department of Chemical Sciences, University of Catania
contact email	vcunsolo@unict.it
lab head
Vincenzo Cunsolo
contact affiliation	University of Catania
contact email	vcunsolo@unict.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078377
     1. Label: PRIDE project
     2. Name: Proteoform analysis by mass spectrometry reveals post-translational processing of legumins and vicilins in chickpeas (Cicer arietinum L.)