PXD078286

PXD078286 is an original dataset announced via ProteomeXchange.

Title	A c-di-AMP receptor mediates epithelial adhesion of Lactiplantibacillus plantarum
Description	Cyclic di-adenosine monophosphate (c-di-AMP) is a crucial second messenger used by bacteria to mediate bacterial-host interactions. However, its roles in Lactiplantibacillus plantarum remain poorly understood. Here, we identify RwpL, which consists solely of an HTH domain, as a c-di-AMP receptor in L. plantarum WCFS1. RwpL mediates epithelial adhesion of L. plantarum WCFS1 and functions as both a transcription inhibitor and activator, with transcriptional output determined by the position of its binding site relative to the transcription start site (TSS) of target genes. Specifically, RwpL inhibits wxlA transcription by binding downstream of its TSS, thus reducing WxlA interactions with fibronectin, a matrix protein on the surface of intestinal epithelia. In contrast, RwpL activates transcription of the matrix-degrading metalloprotease gene mmpL by recruiting the sigma factor RpoA upstream of its TSS. Notably, elevated c-di-AMP levels diminish RwpL binding affinity for both DNA and RpoA, thereby reversing its regulatory functions and enhancing L. plantarum adhesion to host epithelial cells. Together, our findings uncover a dual and reversible regulatory mechanism through which c-di-AMP modulates bacterial-host interactions in L. plantarum.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-25_03:55:45.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	quan Guo
SpeciesList	scientific name: Lactobacillus plantarum WCFS1; NCBI TaxID: NEWT:220668;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-13 13:10:46	ID requested
⏵ 1	2026-05-25 03:55:46	announced

Dataset with its publication pending

submitter keyword: Colonization, c-di-AMP,Lactiplantibacillus plantarum

Quan Guo
contact affiliation	Hainan University
contact email	guoq@hainanu.edu.cn
lab head
quan Guo
contact affiliation	Hainan University
contact email	guoq@hainanu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078286
     1. Label: PRIDE project
     2. Name: A c-di-AMP receptor mediates epithelial adhesion of Lactiplantibacillus plantarum