PXD078272

PXD078272 is an original dataset announced via ProteomeXchange.

Title	Research data to support manuscript titled: Mapping Phosphorylation-Specific Pin1-CRMP2 Interactions using an Integrated Mass Spectrometry Approach
Description	ABSTRACT: Abnormal protein phosphorylation is a fundamental trigger in the pathogenesis of Alzheimer’s Disease, leading to the formation of neurofibrillary tangles. Thus, molecular determination of the critical factors in controlling phosphorylation is desirable. Pin1, a cis-trans prolyl isomerase has recently been implicated in Alzheimer’s Disease progression. Moreover, Pin1 specifically targets phosphoproteins, regulating their function. Here, we reveal a novel interaction interface between Pin1 and the Collapsin Response Mediator Protein-2 (CRMP2); a protein found hyperphosphorylated alongside tau within neurofibrillary tangles. Using native mass spectrometry, we show that Pin1 binds to the disordered C-terminus of CRMP2 in a phosphorylation-dependent manner with residues Thr509 and Thr514 on CRMP2 important for enhanced binding affinity. Hydrogen-deuterium exchange mass spectrometry experiments further localized this binding site to the WW-domain of Pin1. Together, these findings provide novel insight into a putative regulatory role of Pin1 in modulating hyper-phosphorylation of CRMP2.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_05:48:27.085.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078272
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aneika Leney
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-13 07:19:10	ID requested
⏵ 1	2026-05-20 05:48:27	announced

10.6019/PXD078272;

10.1021/ACSCHEMBIO.6C00227;

submitter keyword: native mass spectrometry, hydrogen-deuterium exchange mass spectrometry, protein-ligand binding,peptidyl prolyl isomerase

Aneika Leney
contact affiliation	School of Biosciences, University of Birmingham, UK
contact email	a.leney@bham.ac.uk
lab head
Aneika Leney
contact affiliation	University of Birmingham
contact email	a.leney@bham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD078272

PRIDE project URI

• PRIDE
  1. PXD078272
     1. Label: PRIDE project
     2. Name: Research data to support manuscript titled: Mapping Phosphorylation-Specific Pin1-CRMP2 Interactions using an Integrated Mass Spectrometry Approach