⮝ Full datasets listing
PXD078228
PXD078228 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DIA-Based Quantitative Proteomic Dataset of SH-SY5Y Cells Overexpressing Wild-Type and SAP-Domain Deleted MKL2 |
| Description | This project provides a comprehensive quantitative proteomics dataset generated from human neuroblastoma SH-SY5Y cells to investigate the regulatory roles of MKL2 and its SAP domain. The study involves three experimental groups with three biological replicates each (9 samples in total). SH-SY5Y cells were transiently transfected with an empty vector (Control), wild-type MKL2 (Full-length), or a mutant MKL2 lacking the SAP domain. The raw mass spectrometry files are named as follows: (1) Control group: vector1-3.raw; (2) Wild-type MKL2 group: M_FLOE1-3.raw; (3) SAP-domain deleted MKL2 group: MSAPOE1-3.raw. Cells were harvested 48 to 72 hours post-transfection, and total proteins were extracted using SDT lysis buffer (4% SDS, 100 mM DTT, 100 mM Tris-HCl, pH 8.0). Protein digestion was performed using the Filter-Aided Sample Preparation (FASP) method. The resulting peptides were analyzed on an Orbitrap Astral mass spectrometer coupled with a Vanquish Neo UHPLC system in data-independent acquisition (DIA) mode (survey scan: 380–980 m/z; MS/MS scan: 150–2000 m/z). Raw data files were initially processed using DIA-NN software (version 1.8.1) searched against the UniProtKB Homo sapiens database for quality control and protein identification. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-11 |
| AnnouncementXML | Submission_2026-05-11_00:33:03.129.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xue Chen |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-11 00:28:46 | ID requested | |
| ⏵ 1 | 2026-05-11 00:33:03 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SH-SY5Y cells,MKL2,SAP domain,Data-Independent Acquisition (DIA),Orbitrap Astral,Raw Data |
Contact List
| Guojun Chen | |
|---|---|
| contact affiliation | The First Affiliated Hospital of Chongqing Medical University |
| contact email | 1009205828@qq.com |
| lab head | |
| Xue Chen | |
| contact affiliation | The First Affiliated Hospital of Chongqing Medical University |
| contact email | 2022320009@stu.cqmu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
