PXD078219

PXD078219 is an original dataset announced via ProteomeXchange.

Title	PAR3-mediated coordination of hepatocyte proliferation, maturation, and architecture in liver development and regeneration
Description	The liver’s ability to coordinate cell proliferation, polarization, and maturation with architectural assembly is fundamental to its metabolic functions and regenerative capacity. However, the mechanisms that integrate these processes remain incompletely understood. Using murine and human models, we show that hepatic polarity and bile canaliculi (BC) formation initiate at sites of cell division during the hepatoblast stage, and that BC elongation is temporally associated with oriented cell division during hepatocyte differentiation. This process involves PAR3- and YAP-dependent modulation of ERK/MAPK signaling. PAR3 forms a protein complex that localizes a subset of IQGAP1 to tight junctions, thereby restricting ERK/MAPK activation at the basolateral membrane. This regulation is required for efficient BC elongation and hepatocyte maturation. Together, these findings reveal a stage-dependent mechanism linking liver architecture to functional maturation and provide insights into liver development, regeneration, and potential therapeutic strategies for metabolic liver disease and liver failure.
HostingRepository	iProX
AnnounceDate	2026-05-10
AnnouncementXML	Submission_2026-05-10_20:27:53.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengran Xu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-10 20:27:27	ID requested
⏵ 1	2026-05-10 20:27:55	announced

Dataset with its publication pending

submitter keyword: PAR3, IQGAP1, ERK1/2, liver polarity

Chengran Xu
contact affiliation	Peking University
contact email	cxu@pku.edu.cn
lab head
Chengran Xu
contact affiliation	School of Basic Medical Sciences, Peking University
contact email	cxu@pku.edu.cn
dataset submitter

iProX dataset URI