PXD078215

PXD078215 is an original dataset announced via ProteomeXchange.

Title	Endogenous Sulfane Sulfur Mediates the Oxidative Stress Response Process in Pseudomonas aeruginosa
Description	Sulfane sulfur species are increasingly recognized as integral cellular components involved in sig-naling pathways and cytoprotection against oxidative stress in mammals. While their production in bacteria has been extensively studied, their functional role in bacterial oxidative stress defense re-mains poorly understood. Here, we demonstrate that sulfane sulfur generated by sulfide:quinone oxidoreductase reduces the sensitivity to H2O2 in P. aeruginosa PAO1. Notably, this protective mechanism does not depend on sulfane sulfur acting as a direct H2O2 scavenger via electrophilic reactions. Through persulfidated proteomic profiling, we reveal that persulfidation is a prominent post-translational modification of P. aeruginosa, reflecting the prevalence of deprotonated sulfane sulfur species. These species modify cysteine residues from proteins, including the well-known oxidative stress regulator OxyR. Specifically, sulfane sulfur modifies OxyR at Cys199 to form a persulfidated OxyR C199-SSH, endowing a single-Cys activated state that modulates promoter ac-tivities and DNA-binding affinity. Furthermore, persulfidation mediated by sulfane sulfur protects the critical cysteine residue of LpdG, a ROS-vulnerable glycolytic enzyme, from irreversible hy-peroxidation. Although LpdG is not part of the canonical H2O2 scavenging system, its preservation is essential for cell viability under oxidative stress. These findings establish endogenous sulfane sulfur as key mediators of antioxidant defenses in P. aeruginosa.
HostingRepository	iProX
AnnounceDate	2026-05-09
AnnouncementXML	Submission_2026-05-10_20:17:50.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weining Sun
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-10 20:17:10	ID requested
⏵ 1	2026-05-10 20:17:51	announced

Dataset with its publication pending

submitter keyword: Pseudomonas aeruginosa, Oxidative Stress, Sulfane Sulfur

Weining Sun
contact affiliation	Hangzhou Normal University
contact email	20220150@hznu.edu.cn
lab head
Weining Sun
contact affiliation	Hangzhou Normal University
contact email	20220150@hznu.edu.cn
dataset submitter

iProX dataset URI