PXD078173

PXD078173 is an original dataset announced via ProteomeXchange.

Title	Role of Type VI Secretion System Core Proteins Hcp and VgrG in Antibiotic Resistance of Acinetobacter baumannii
Description	The antimicrobial resistance of Acinetobacter baumannii (A. baumannii) is becoming increasingly severe. This study aimed to investigate the role and mechanism of the type VI secretion system (T6SS) core protein hemolysin coregulated protein (Hcp) and valine-glycine repeat protein G (VgrG) in antibiotic resistance regulation in Acinetobacter baumannii. Using ATCC 17978 as the parental strain, we constructed Δhcp, ΔvgrG3, and ΔhcpΔvgrG3 knockout mutants. Transcriptomic and proteomic analyses, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation, and antibiotic susceptibility testing (AST) in the presence or absence of the efflux pump inhibitor phenylalanine-arginine β-naphthylamide (PAβN) were performed. The results showed that the Δhcp mutant primarily downregulated pathways related to amino acid metabolism, secretion systems, and biofilm formation, with no significant changes in the minimum inhibitory concentrations (MICs) against all tested antibiotics. In contrast, the ΔvgrG3 mutant significantly upregulated the resistance-nodulation-division (RND) efflux pump AdeIJK, as well as pathways associated with resistance to β-lactam antibiotics and cationic antimicrobial peptides, resulting in broadly elevated MICs. The double knockout mutant ΔhcpΔvgrG3 further downregulated adeN, leading to enhanced AdeIJK expression. It also synergistically regulated major facilitator superfamily (MFS) efflux pumps, and lipopolysaccharide biosynthesis, causing further increases in resistance to piperacillin/tazobactam, ceftazidime, cefepime, and meropenem. Treatment with the efflux pump inhibitor PAβN partially reversed the resistance phenotypes of the mutants. In conclusion, Hcp and VgrG3 exhibit distinct functional differentiation in the regulation of antibiotic resistance in A. baumannii. Hcp has a limited impact on resistance, whereas VgrG3 critically contributes to multidrug resistance by negatively regulating the expression of the AdeIJK efflux pump system.
HostingRepository	iProX
AnnounceDate	2026-05-08
AnnouncementXML	Submission_2026-05-08_18:09:56.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meiyuan Huang
SpeciesList	scientific name: Acinetobacter baumannii; NCBI TaxID: 470;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-08 18:09:34	ID requested
⏵ 1	2026-05-08 18:09:57	announced

Dataset with its publication pending

submitter keyword: Acinetobacter baumannii, hemolysin co-regulated protein, valine-glycine repeat protein G, type VI secretion system, AdeIJK efflux pump, antibiotic resistance

Daojun Yu
contact affiliation	Hangzhou First People's Hospital
contact email	yudaojun@hospital.westlake.edu.cn
lab head
Meiyuan Huang
contact affiliation	Hangzhou First People's Hospital
contact email	18508485063@163.com
dataset submitter

iProX dataset URI